Current Issue : July - September Volume : 2017 Issue Number : 3 Articles : 6 Articles
To explore the mechanisms by which andrographolide inhibits gastric cancer cell proliferation and metastasis, we employed the\ngastric cell line SGC7901 to investigate the anticancer effects of andrographolide.The cell survival ratio, cell migration and invasion,\ncell cycle, apoptosis, and matrix metalloproteinase activity were assessed. Moreover, western blotting and real-time PCR were\nused to examine the protein expression levels and the mRNA expression levels, respectively. The survival ratio of cells decreased\nwith an increasing concentration of andrographolide in a dose-dependent manner. Consistent results were also obtained using an\napoptosis assay, as detected by flow cytometry.The cell cycle was blocked at the G2/M2 phase by andrographolide treatment, and\nthe proportion of cells arrested at G1/M was enhanced as the dose increased. Similarly, wound healing and Transwell assays showed\nreduced migration and invasion of the gastric cancer cells at various concentrations of andrographolide. Andrographolide can\ninhibit cell proliferation, invasion, and migration, block the cell cycle, and promote apoptosis in SGC7901 cells. The mechanisms\nmay include upregulated expression of Timp-1/2, cyclin B1, p-Cdc2, Bax, and Bik and downregulated expression of MMP-2/9 and\nantiapoptosis protein Bcl-2....
Objective: To detect the expression and pathological significances of Interleukin-\n6 (IL-6) and basic fibroblast growth factor (bFGF) in tongue squamous\ncell carcinoma (TSCC). Methods: A tissue array was analyzed immunohistochemically.\nThe expression levels of IL-6 and bFGF in tissues were recorded\nsemi-quantitatively. Results: The positive expression of IL-6 in normal, benign\ntumor, and carcinoma groups was 12.50% �± 5.575%, 39.00% �± 1.41%,\n77.26% �± 17.07% respectively, and the expression among the three groups\nshowed significant differences (P < 0.05). The expression of bFGF in benign\ntumor was lower than that in normal groups and squamous cell carcinoma.\nThe positive expression was 6.50% �± 2.12%, 23.75% �± 4.79%, 42.33% �±\n29.89% respectively, and the expression among the three groups showed significant\ndifferences (P < 0.05). Furthermore, there were positive correlations\nbetween IL-6 and bFGF (P < 0.05). Conclusion: It indicates that combining\nimmunohistochemical examination of IL-6 and bFGF has an important reference\nvalue on the pathological diagnosis of tongue squamous cell carcinoma....
Background: Head and neck squamous cell carcinoma (HNSCC) represents a unique and major health concern\nworldwide. Significant increases in glucose uptake and aerobic glycolysis have been observed in HNSCC cells.\nGlucose transporters (GLUTs) represent a major hub in the glycolysis pathway, with GLUT4 having the highest\nglucose affinity. However, GLUT4ââ?¬â?¢s role in HNSCC has not been fully appreciated.\nMethods: An in silico analysis was performed in HNSCC cohorts to identify the most significant glucose transporter\nassociated with HNSCC patient prognosis. An immunohistochemical analysis of a tissue microarray with samples\nfrom 90 HNSCC patients was used to determine the association of GLUT4 with prognosis. Complementary functional\nexpression and knockdown studies of GLUT4 were performed to investigate whether GLUT4 plays a role in HNSCC cell\nmigration and invasion in vitro and in vivo. The detailed molecular mechanism of the function of GLUT4 in inducing\nHNSCC cell metastasis was determined.\nResults: Our clinicopathologic analysis showed that increased GLUT4 expression in oral squamous cell carcinoma\npatients was significantly associated with a poor overall survival (OS, P = 0.035) and recurrence-free survival (RFS, P = 0.\n001). Furthermore, the ectopic overexpression of GLUT4 in cell lines with low endogenous GLUT4 expression resulted\nin a significant increase in migratory ability both in vitro and in vivo, whereas the reverse phenotype was observed in\nGLUT4-silenced cells. Utilizing a GLUT4 overexpression model, we performed gene expression microarray and Ingenuity\nPathway Analysis (IPA) to determine that the transcription factor tripartite motif-containing 24 (TRIM24) was the main\ndownstream regulator of GLUT4. In addition, DDX58 was confirmed to be the downstream target of TRIM24, whose\ndownregulation is essential for the migratory phenotype induced by GLUT4ââ?¬â??TRIM24 activation in HNSCC cells.\nConclusions: Here, we identified altered glucose metabolism in the progression of HNSCC and showed that it could\nbe partially attributed to the novel link between GLUT4 and TRIM24. This novel signaling axis may be used for the\nprognosis and therapeutic treatment of HNSCC in the future...
We aimed to identify prognostic factors associated with progression-free survival (PFS) and overall survival (OS) in metastatic\nrenal cell carcinoma (mRCC) patients treated with sorafenib. We investigated 177 patients, including 116 who received sorafenib\nas first-line therapy, using the Cox regression model. During a median follow-up period of 19.2 months, the PFS and OS were 6.4\nand 32.6 months among all patients and 7.4 months and undetermined for first-line sorafenib-treated patients, respectively. Clinical\nT3-4 stage (hazard ratio [HR] 2.56) and a primary tumor size >7 cm(HR 0.34) were significant prognostic factors for PFS among all\npatients, as were tumor size >7 cm(HR 0.12), collecting system invasion (HR 5.67), and tumor necrosis (HR 4.11) for OS (...
Background: Cervical cancer (CC) occupies fourth place in cancer incidence and mortality worldwide in women,\nwith 560,505 new cases and 284,923 deaths per year. Approximately, nine of every ten (87%) take place in developing\ncountries. When a macroscopic nodal involvement is discovered during a radical hysterectomy (RH), there is\ncontroversy in the literature between resect macroscopic lymph node compromise or abandonment of the\nsurgery and sending the patient for standard chemo-radiotherapy treatment. The objective of this study is to\ncompare the prognosis of patients with CC whom RH was abandoned and bilateral pelvic lymphadenectomy\nand para-aortic lymphadenectomy was performed with that of patients who were only biopsied or with removal of a\nsuspicious lymph node, treated with concomitant radiotherapy/chemotherapy in the standard manner.\nMethods: A descriptive and retrospective study was conducted in two institutions from Mexico and Colombia. Clinical\nrecords of patients with early-stage CC programmed for RH with an intraoperative finding of pelvic lymph, para-aortic\nnodes, or any extracervical involvement that contraindicates the continuation of surgery were obtained. Between\nJanuary 2007 and December 2012, 42 clinical patients complied with study inclusion criteria and were selected\nfor analysis.\nResults: In patients with CC whom RH was abandoned due to lymph node affectation, there is no difference in\noverall survival or in disease-free period between systematic lymphadenectomy and tumor removal or lymph\nnode biopsy, in pelvic lymph nodes as well as in para-aortic lymph nodes, when these patients receive adjuvant\ntreatment with concomitant radiotherapy/chemotherapy.\nConclusions: This is a hypothesis-generator study; thus, the recommendation is made to conduct randomized\nprospective studies to procure better knowledge on the impact of bilateral pelvic and para-aortic\nlymphadenectomy on this group of patients....
Background. The most common malignant tumor of the urinary bladder is transitional cell carcinoma (TCC). Neural precursor\ncell-expressed developmentally downregulated protein 9 (NEDD9) is found to be a cell adhesion mediator. P38Mitogen-Activated\nProtein Kinase is a serine/threonine kinases member which can mediate carcinogenesis through intracellular signaling. Methods.\nTo assess their prognostic role; NEDD9 and p38 protein were evaluated in sections from 50 paraffin blocks of TCC. Results. The\nhigh expressions of NEDD9 and p38 protein were significantly associated with grade, stage, distant metastasis (...
Loading....